He said on Wednesday in Freetown that Dr Michael Kargbo died on Tuesday afternoon.
He said it was not clear how Kargbo, a 64-year-old dermatologist working at the Magburaka Government Hospital, was infected with Ebola as he was not serving in a frontline Ebola treatment unit.
He noted that more than 128 of Sierra Leonean health personnel infected by Ebola as staff working in general wards and special treatment centres have been exposed to the disease.
He said all seven Sierra Leonean doctors who have contracted Ebola have died.
The worst Ebola outbreak on record has killed over 5,000 people, mainly in Guinea, Liberia and Sierra Leone.
However, UN officials said the disease was advancing rapidly in Sierra Leone, where there is a lack of treatment centers.
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